The TYGS is currently limited to 50 genomes not because of a limitation of the method but because we have to keep an eye on the compute cluster usage. But for most use cases this limit is clearly more than enough. However, we usually offer to increase the upload cap for a given email address on TYGS beyond the default of 50 user genomes when asked for, assuming the requested upload cap is not exorbitantly high.
Before asking for an increased upload cap, please consider and prepare the following aspects though:
How does the procedure work?
Please use the TYGS feedback form and answer the above questions as complete as possible. Upon reception of your request, we will check it and might come back to you with further questions (basically to narrow down the computational complexity of your specific job). If everything is fine, we will set up an exception for your e-mail address which allows you to submit TYGS requests with the requested number of user sequences. Should the web server dislike a larger amount of data and produces a timeout, we can usually offer to submit the files on your behalf from within our network. In that case the file exchange can be be organized via a confidential folder on our institutional cloud.
The exception will be usually valid for a week but can normally be extended on request. Please note that your job(s) will use some resources of our in-house compute cluster. That is, please do not start multiple redundant jobs of that size if this can be avoided.