Type Strain Genome Server

News TYGS has completed 100,000 jobs
We are delighted to announce that TYGS has delivered 100,000 jobs to date. We thank all users for their confidence in TYGS and hope to serve the community in the future.
News Extended 16S ML/MP analyses are now displayed within the TYGS
Previously, the extended 16S ML/MP analyses were ordered by the user on the respective TYGS job result page but the results were only made available through the GGDC phylogeny server via email. To further streamline the interaction with the various results, we have now integrated the extended 16S ML/MP analyses into the TYGS result page. Since the main purpose of the extended 16S ML/MP analyses is to detect not yet genome-sequenced type strains, it is now much easier to cross compare the various result phylogenies.
News TYGS Year 2022 in Review report released in both PDF and PNG format
News Increased default upload cap for user genome files

We have increased the default upload cap from 20 to 50 genome sequences. In case you still need larger analyses, please refer to the TYGS FAQ. Please note, the TYGS uses a computing infrastructure to process your request but computing resources are finite and have to be shared among many users across the world. That is, please try to avoid redundant analyses, i.e., please avoid multiple analyses that basically use the same dataset. In case you are unsure on how to use the TYGS, please use the feedback form.

News TYGS Year 2021 in Review report released in both PDF and PNG format
News New paper on the TYGS and LPSN database tandem published in Nucleic Acids Research

Our new paper describing the relationship between the databases of the Type (Strain) Genome Server (TYGS) and the List of Prokaryotic names with Standing in Nomenclature (LPSN) was recently published in Nucleic Acids Research:

TYGS and LPSN: a database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes.

Change Increased retention time for user jobs from 2 weeks to 4 weeks

As you might have noticed, the TYGS will remove old job results mainly due to data privacy reasons. The old job result lifetime was 2 weeks and was now extended to 4 weeks. Apart from that, whenever you need your job results a bit longer, just use the contact form to send us a short request and we will extend the lifetime of your job.

News TYGS Year 2020 in Review report released in both PDF and PNG format
Change Faster javascript plugin for manual selection of type strain genomes
[CHANGED] TYGS now uses a much faster and more convenient multi select javascript plugin called virtual-select.
Change Reconstruction of proteome-based GBDP trees
[ADDED] For very diverse datasets of strains, the average branch support, even of the genome-based phylogeny, might be too low which is not unlikely for such datasets. An optional proteome-based GBDP analysis will become available on user request if the dataset is not too large (< 30 strains) and the average branch support of the genome-based tree is smaller than 50%.
Change Extended 16S rRNA gene sequence analysis now available from main TYGS result page
[CHANGED] The extended SSU analysis will now be found on the main TYGS result page and not longer on the subpage of the 16S GBDP phylogeny.
News Several milestones reached
~7,000,000 total number of pairwise genome comparisons
~600,000 average number of pairwise genome comparisons per month
>13,000 total number of TYGS analyses so far
>2,000 total number of distinct scientists from all around the world so far
~300 average number of distinct scientists per month
~130 average number of first-time users per month
Change GBDP core
[CHANGED] A routine was replaced in the coverage algorithm of the GBDP core, with the newer one being a bit more complex but better able to deal with highly similar genome sequences that significantly differ in length and are highly redundant e.g. due to sequencing or assembly artifacts.
Change Splitting of user requests
[ADDED] Sometimes job submissions contain user genomes or metagenomic bins that are only remotely related, if at all. But a single TYGS analysis including all of these genomes at once would then result in a 'bloated' user request analysis when being subjected to the automatic determination of closest type strain neighbors. In such cases the TYGS now splits your request into two or more sub requests reflecting groups of genomic relatedness. the job confirmation page now also holds an overview of the status of these forked requests and a direct link to the individual result pages. This feature further improves your genome-based taxonomic analyses by finding an even more suitable type strain selection.
News Preferred names of type strains now link to the rebooted LPSN page
Since February 2020 the LPSN page (List of Prokaryotic names with Standing in Nomenclature) is now officially hosted, developed and maintained at the Leibniz Institute DSMZ and readily available under https://lpsn.dsmz.de. This affects the TYGS in so far as the preferred name of each type strain listed in Table 4 of your individual TYGS result page now links to the specific LPSN page of that species. Moreover, links to the BacDive database now go to the specific strain page whenever possible.
Change Compact PDF report of your individual TYGS result page
[ADDED] Since December 2019 the TYGS also offers the download of a nice publication-ready PDF report which can, in principle, be added to a paper as a supplement.
Change Genome-based approach to determine closest type strain genomes
[CHANGED] The most closely related type-strain genomes are now also determined independently of 16S rRNA gene sequences. In addition to the ten closest type-strain genomes determined via the smallest 16S rRNA sequence distances, the TYGS now also calculates intergenomic MASH distances (PubMed ID: 27323842), a fast approximation of intergenomic relatedness, between each uploaded genome sequence and the entire set of type strain genomes contained in the TYGS database. The set of type-strain genomes with the smallest MASH distances is combined with those having the smallest 16S rRNA gene distances. This approach ensures that even if the user genome sequence does not contain a 16S rRNA gene and even in organismal groups in which the 16S rRNA gene sequence does not well resolve the pairwise relationships, the most closely related type strains are chosen. Note that the usage of 16S rRNA gene sequences remains relevant for detecting closely related type strains that have not yet been genome-sequenced.
Change Tree export to PNG format
[FIXED] The legend text is no longer cut off at the top when exporting trees to PNG format.
Change Quality criteria for the import of reference genomes
[CHANGED] New type(-strain) reference genome sequences are only included in the TYGS database, if certain quality criteria are met. The latter were recently adjusted, i.e., now genomes with >500 contigs are accepted if the annotation contains a 16S rRNA gene sequence. When introduced this change resulted in the addition of only few dozens of new genome sequences.