Increased default upload cap for user genome files
We have increased the default upload cap from 20 to 50 genome sequences. In case you still need
larger analyses, please refer to the TYGS FAQ.
Please note, the TYGS uses a computing infrastructure to process your request but
computing resources are finite and have to be shared among many users
across the world. That is, please try to avoid redundant analyses, i.e.,
please avoid multiple analyses that basically use the same dataset. In case you are
unsure on how to use the TYGS, please use the feedback form.
TYGS Year 2021 in Review report released in both PDF and PNG format
Increased retention time for user jobs from 2 weeks to 4 weeks
As you might have noticed, the TYGS will remove old job results mainly due to data privacy reasons. The old job result lifetime was 2 weeks and was now extended to 4 weeks.
Apart from that, whenever you need your job results a bit longer, just use the contact form to send us a short request and we will extend the lifetime of your job.
TYGS Year 2020 in Review report released in both PDF and PNG format
Extended 16S rRNA gene sequence analysis now available from main TYGS result page
[CHANGED] The extended SSU analysis will now be found on the main TYGS result page and not longer on the subpage of the 16S GBDP phylogeny.
Reconstruction of proteome-based GBDP trees
[ADDED] For very diverse datasets of strains, the average branch support, even of the genome-based phylogeny, might be too low which is not unlikely for such datasets. An optional proteome-based GBDP analysis will become available on user request if the dataset is not too large (< 30 strains) and the average branch support of the genome-based tree is smaller than 50%.
Several milestones reached
total number of pairwise genome comparisons
average number of pairwise genome comparisons per month
total number of TYGS analyses so far
total number of distinct scientists from all around the world so far
average number of distinct scientists per month
average number of first-time users per month
[CHANGED] A routine was replaced in the coverage algorithm of the GBDP core, with the newer one being a bit more complex but better able to deal with highly similar genome sequences that significantly differ in length and are highly redundant e.g. due to sequencing or assembly artifacts.
Splitting of user requests
[ADDED] Sometimes job submissions contain user genomes or metagenomic bins that are only remotely related, if at all. But a single TYGS analysis including all of these genomes at once would then result in a 'bloated' user request analysis when being subjected to the automatic determination of closest type strain neighbors. In such cases the TYGS now splits your request into two or more sub requests reflecting groups of genomic relatedness. the job confirmation page now also holds an overview of the status of these forked requests and a direct link to the individual result pages. This feature further improves your genome-based taxonomic analyses by finding an even more suitable type strain selection.
Preferred names of type strains now link to the rebooted LPSN page
Since February 2020 the LPSN page (List of Prokaryotic names with Standing in Nomenclature) is now officially hosted, developed and maintained at the Leibniz Institute DSMZ and readily available under https://lpsn.dsmz.de. This affects the TYGS in so far as the preferred name of each type strain listed in Table 4 of your individual TYGS result page now links to the specific LPSN page of that species. Moreover, links to the BacDive database now go to the specific strain page whenever possible.
Compact PDF report of your individual TYGS result page
[ADDED] Since December 2019 the TYGS also offers the download of a nice publication-ready PDF report which can, in principle, be added to a paper as a supplement.
Genome-based approach to determine closest type strain genomes
[CHANGED] The most closely related type-strain genomes are now also determined independently of 16S rRNA gene sequences. In addition to the ten closest type-strain genomes determined via the smallest 16S rRNA sequence distances, the TYGS now also calculates intergenomic MASH distances (PubMed ID: 27323842), a fast approximation of intergenomic relatedness, between each uploaded genome sequence and the entire set of type strain genomes contained in the TYGS database. The set of type-strain genomes with the smallest MASH distances is combined with those having the smallest 16S rRNA gene distances. This approach ensures that even if the user genome sequence does not contain a 16S rRNA gene and even in organismal groups in which the 16S rRNA gene sequence does not well resolve the pairwise relationships, the most closely related type strains are chosen. Note that the usage of 16S rRNA gene sequences remains relevant for detecting closely related type strains that have not yet been genome-sequenced.
Tree export to PNG format
[FIXED] The legend text is no longer cut off at the top when exporting trees to PNG format.
Quality criteria for the import of reference genomes
[CHANGED] New type(-strain) reference genome sequences are only included in the TYGS database, if certain quality criteria are met. The latter were recently adjusted, i.e., now genomes with >500 contigs are accepted if the annotation contains a 16S rRNA gene sequence. When introduced this change resulted in the addition of only few dozens of new genome sequences.